| Organised by BITS, the Bioinformatics Training and Service facility of the VIB. |
 |
Goal
- Obtain a good understanding of the origins and properties of mass spec data
- Obtain an understanding of the processing of mass spec data, aimed at identifying peptides and proteins
- Gain sufficient understanding of the software tools and database used, and of the issues and caveats involved, to critically analyse and assess results from mass spectrometry based proteomics experiments
Summary
In this training session we start by reviewing the types of mass spectrometer, and what the properties of their output data are. Out of our understanding of the data, we learn to extract meaningful interpretations.
Required skills
A basic understanding of mass spectrometry as applied to peptides and proteins is required by the participants.
Detailed schedule
| 09h00-09h45 |
Lecture |
Mass spectrometry basics |
Lennart Martens |
| 09h45-10h30 |
Lecture |
Introduction to proteomics |
Kenny Helsens
|
| 10h30-10h45 |
Coffee break |
|
| 10h45-11h45 |
Lecture |
Search engines |
Lennart Martens |
| 11h45-12h15 |
Lecture |
Sequence databases |
Lennart Martens |
| 12h15-13h15 |
Lunch |
|
| 13h15-14h15 |
Practical |
Using X!Tandem and OMSSA through SearchGUI |
Lennart and Kenny |
| 14h15-15h15 |
Lecture |
Peptide identification validation and protein inference |
Kenny Helsens |
| 15h15-15h30 |
Coffee break |
|
| 15h30-17h00 |
Practical |
PeptideShaker to browse, analyze and interpret search results |
Lennart and Kenny |
Trainer
This training session is prepared and given by Lennart Martens and Kenny Helsens, members of the CompOmics lab at VIB.