Transgenic Mouse Core Facility

Research focus

​The Transgenic mouse Core Facility (TmCF) of the IRC was established in 2004 to cater to the needs of IRC investigators. The mission of the TmCF is to provide efficient and economic access to transgenic animals and related technologies.

Summary of services
Gene targeting in ES-cells
Embryonic Stem (ES) cells are electroporated with a targeting vector designed to introduce a specific mutation in the mouse genome. After antibiotic selection, surviving colonies are picked and expanded for screening and freezing. To generate transgenic mice over-expressing a gene of interest, dedicated ES-cells and targeting vectors are available to specifically introduce the transgene in the Rosa26 locus.

Generation of chimeras
Correctly targeted ES cells are introduced in an early mouse embryo. This can be done by injecting the ES cells into blastocysts or by aggregating ES cell clumps with morulas; The ES cells assist in the formation of the fetus. The resulting mouse is a chimera composed of two cell types: cells derived from the embryo and those derived from the introduced ES cells. The chimeric mice are mated with wild-type mice to transmit the desired mutation from the chimeric to the offspring

Generation of mutant mice
Nuclease mediated gene editing is a powerful way for making mutant mice. Crispr/Cas9 technology is very efficient for making knockin and even multiplex knockout mice. The technology also allows for a significant time reduction compared to ES-cell mediated gene targeting and can be used to generate mutant mice in virtually any desired background.

Rederivation of mouse strains
The presence of any type of mouse pathogen (whether it causes clinical disease or not), changes the physiology of the mouse, which can lead to false experimental results. Mouse strains harboring pathogens can be ‘rederived’ to a pathogen-free status by transferring pre-implantation embryos from a contaminated mouse to a pathogen-free foster mother. The resulting pups will then also be pathogen-free.

Cryopreservation
The purpose of cryopreservation is to protect against loss of valuable, unique mouse lines through breeding failure or disease, and to eliminate the cost of maintaining mouse lines that are not in use. Sperm freezing is the default method as it is quick and few mice are needed. An IVF is required with the frozen sperm to revive the mouse line. For complex genetic backgrounds, embryo freezing is the preferred method.

Publications

DMRT5 Together with DMRT3 Directly Controls Hippocampus Development and Neocortical Area Map FormationDe Clercq S, Keruzore M, Desmaris E, Pollart C, Assimacopoulos S, Preillon J, Ascenzo S, Matson C, Lee M, Nan X, Li M, Nakagawa Y, Hochepied T, Zarkower D, Grove E, Bellefroid ECEREBRAL CORTEX, 28, 493-509, 2018
Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activationVan Gorp H* Saavedra P* Vasconcelos N Van Opdenbosch N Vande Walle L Matusiak M Prencipe G Insalaco A Van Hauwermeiren F Demon D Bogaert D Dullaers M De Baere E Hochepied T Dehoorne J Vermaelen K Haerynck F De Benedetti F Lamkanfi MPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113, 14384-14389, 2016* These authors contributed equally
Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in miceThoonen R Cauwels A Decaluwe K Geschka S Tainsh R Delanghe J Hochepied T De Cauwer L Rogge E Voet S Sips P Karas R Bloch K Vuylsteke M Stasch J Van De Voorde J Buys E* Brouckaert P*Nature Communications, 6, 8482, 2015* These authors contributed equally
RNF4 is required for DNA double-strand break repair in vivoVyas R, Kumar R, Clermont F, Helfricht A, Kalev P, Sotiropoulou P, Hendriks I, Radaelli E, Hochepied T, Blanpain C, Sablina A, Van Attikum H, Olsen J, Jochemsen A, Vertegaal A, Marine JCELL DEATH AND DIFFERENTIATION, 20, 490-502, 2013
The ROSA26-iPSC Mouse: A Conditional, Inducible, and Exchangeable Resource for Studying Cellular (De)DifferentiationHaenebalcke L, Goossens S, Dierickx P, Bartunkova S, D'Hont J, Haigh K, Hochepied T, Wirth D, Nagy ACell Reports , 3, 335-41, 2013

Job openings

Tino Hochepied

Tino Hochepied

Model organism(s)

Bio

​PhD: Univ. of Ghent, Ghent, Belgium, 2001
VIB Expert Technologist since 2009

Contact Info

VIB-UGent Center for Inflammation ResearchUGent-VIB Research Building FSVMTechnologiepark 927 9052 GENTRoute description
Leen Vanhoutte

Leen Vanhoutte

Model organism(s)

Bio

PhD: Univ. of Ghent, Ghent, Belgium, 2009
VIB Expert Technologist as of January 2016

Contact Info

VIB-UGent Center for Inflammation ResearchUGent-VIB Research Building FSVMTechnologiepark 927 9052 GENTRoute description