Charlotte Scott lab

Research focus

Our lab is focused on unravelling heterogeneity within the mononuclear phagocyte compartment in health and disease in terms of both phenotype and function in mice and men. To do this, we use state of the art techniques including single cell RNA sequencing, CITE-seq and 28-parameter flow cytometry alongside MNP-subset specific in vivo mouse models including the Clec4F-Cre to target Kupffer cells in the liver, the Xcr1-Cre to target cDC1s and the Fcgr1-Cre to target all macrophages. Currently, we are examining myeloid cell heterogeneity and functions in acute liver injury, infection and non-alcoholic fatty liver disease (NAFLD, funded by ERC-Stg). NAFLD is currently one of the most significant clinical burdens in the western world and is thought to be the main reason for liver transplantation by 2030. Despite this there are no effective treatments currently available. As MNPs have been proposed to play a role in NAFLD progression we hypothesize that correctly identifying and distinguishing between these cell subsets and subsequently examining their specific functions will lead to a better understanding of this disease and may lead to new avenues for therapy. 

Figure below showing the prevalence of resident macrophages or KCs (in purple) across the healthy and NAFLD liver, showing a decrease in NAFLD and the presence of another macrophage subset (Green) in NAFLD. 
Liver Macrophages_Healthy_Charlotte Scottjpg.jpg   Liver Macrophages_NAFLD_Charlotte scott.jpg
                                Healthy                                                          NAFLD


Research Group
Anna Bujko (postdoc) - Macrophages in acute liver Injury
Christian Zwicker (postdoc) - Macrophages in infection
Kathryn Waller (postdoc) - Dendritic cells in NAFLD
Anneleen Remmerie (PhD student) - Macrophages in NAFLD
Tinne Thone (Bioinformatician Technician)
Tineke Vanhaelewyn (Technician)
Mushida Binte Abdullatib (Technician)

Publications

The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of MacrophagesScott C* T'jonck W* Martens L Todorov H Sichien D Soen B Bonnardel J De Prijck S Vandamme N Cannoodt R Saelens W Vanneste B Toussaint W De Bleser P Takahashi N Vandenabeele P Henri S Pridans C Hume D Lambrecht B De Baetselier P Milling S Van Ginderachter J Malissen B Berx G Beschin A* Saeys Y* Guilliams M*IMMUNITY, 49, 312-325 e5, 2018* or °: authors contributed equally
Does niche competition determine the origin of tissue-resident macrophages?Guilliams M* Scott C*NATURE REVIEWS IMMUNOLOGY, 17, 451-460, 2017* or °: authors contributed equally
Unsupervised High-Dimensional Analysis Aligns Dendritic Cells across Tissues and SpeciesGuilliams M* Dutertre C Scott C Mcgovern N Sichien D Chakarov S Van Gassen S Chen J Poidinger M De Prijck S Tavernier S Low I Irac S Mattar C Sumatoh H Low G Chung T Chan D Tan K Hon T Fossum E Bogen B Choolani M Chan J Larbi A Luche H Henri S Saeys Y Newell E Lambrecht B* Malissen B* Ginhoux F*IMMUNITY, 45, 669-84, 2016* or °: authors contributed equally
Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cellsScott Charlotte Zheng Fang De Baetselier Patrick Martens Liesbet Saeys Yvan De Prijck Sofie Lippens Saskia Abels Chloé Schoonooghe Steve Raes Geert Devoogdt N Lambrecht Bart Beschin Alain Guilliams MartinNature Communications, 7, 10321, 2016
The transcription factor Zeb2 regulates development of conventional and plasmacytoid DCs by repressing Id2Scott C* Soen B* Martens L Skrypek N Saelens W Taminau J Blancke G Van Isterdael G Huylebroeck D Haigh J Saeys Y Guilliams M Lambrecht B* Berx G*JOURNAL OF EXPERIMENTAL MEDICINE, 213, 897-911, 2016* or °: authors contributed equally

Job openings

Charlotte Scott

Charlotte Scott

Research area(s)

Model organism(s)

Bio

PhD: 'Characterization of Dendritic Cells in the Intestine' University of Glasgow, Institute of Infection, Inflammation and Immunity, Supervised by Prof. Allan Mowat. 2010-2014
Postdoctoral Fellow: VIB-UGent Center for Inflammation Research, 2014-2019, Supported by Horizon 2020: Marie Curie IEF, Wellcome Trust: Sir Henry Wellcome Fellowship - Joint with University of Glasgow.

Contact Info

VIB-UGent Center for Inflammation ResearchUGent-VIB Research Building FSVMTechnologiepark 71 9052 GENTRoute description