Within the framework of an ERC Consolidator project, An initial 2-year postdoctoral position is available in the Advanced Live Cell Imaging group of Daniel Van Damme at The VIB/UGent Department of Plant Systems Biology to investigate the ultrastructure of the plant endocytic TPLATE complex (TPC).
The TPC has its evolutionary origin in between the emergence of COPI and the functional specification of the AP complexes. While the TPC remained essential for plants, it was evolutionary lost in yeasts and animal cells, indicating that endocytosis operates differently in plants (Gadeyne et al., Cell 2014: Hirst et al., Elife 2014).
The project aims to generate structural insight into membrane recruitment and cargo identification of the TPC as well as its interplay with the evolutionary conserved AP-2 complex. It will combine structural biology approaches including complex purification from plants, yeast, E. coli and insect cells with single particle EM imaging (negative stain and cryo-EM). The project will run in close collaboration with structural biology groups within the VIB institute (Savvas Savvides, VIB/UGent and Rouslan Efremov, VIB/VUB) and will therefore have access to a plethora of structural biology techniques. It will also be able to benefit from the possibilities of the European Instruct structural biology platform (https://www.structuralbiology.eu/).