Within the framework of an ERC Consolidator project, An initial 2-year postdoctoral position is available in the Advanced Live Cell Imaging group of Daniel Van Damme at The VIB/UGent Department of Plant Systems Biology to investigate cargoes and interactors of the plant endocytic TPLATE complex (TPC).
The TPC has its evolutionary origin in between the emergence of COPI and the functional specification of the AP complexes. While the TPC remained essential for plants, it was evolutionary lost in yeasts and animal cells, indicating that endocytosis operates differently in plants (Gadeyne et al., Cell 2014: Hirst et al., Elife 2014). The project aims to identify transmembrane proteins as cargoes of the TPC as well as other interactors which modulate its activity. It will combine proteomics (TAP-tag, GFP-Trap, Bio-ID, chemical crosslinking) in Arabidopsis seedlings and cell culture cells with higher-order library-based interaction screening in yeast. Identified interactors will be validated and characterized using fluorescence microscopy-based cell-biological assays.
The successful candidate will become part of a lively and international research group with a long track record on unravelling the role of this key endocytic complex in plants (Van Damme et al., Plant Journal 2004; Van Damme et al., Plant Cell 2006; Van Damme et al PNAS 2011; Gadeyne et al., Cell 2014) and will benefit from the in-house availability and extensive know-how in plant proteomics through a long-standing collaboration with the Functional Interactomics group of Prof. Dr. Geert De Jaeger.