A CUG codon adapted two-hybrid system for Candida albicans

Summary of the invention


The use of Saccharomyces cerevisae as a host for yeast two-hybrid experiments with Candida albicans proteins is limited to due erroneous translation caused by the aberrant codon usage of Candida albicans (translation of the CUG codon into serine instead of leucine). VIB researchers constructed a two-hybrid system with Candida albicans as the host with components that are compatible for use in this organism.


Further reading: the functionality of this two-hybrid system was validated in Stynen et al (2010) A CUG codon adapted two-hybrid system of the pathogenic fungus Candida albicans. Nucleic Acids Research. ahead of print. PMID: 20719741



This two-hybrid system can be used to study protein-protein interactions in Candida species. 

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